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Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

《农业科学与工程前沿（英文）》 2015年 第2卷 第3期   页码 260-265 doi: 10.15302/J-FASE-2015070

摘要： Y73 is a progeny of asymmetric somatic hybridization between cv. Dalixiang and the wild rice species . Inoculation with a range of strains of pv. showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent. An F population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24. After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism between Y73 and IR24. Molecular markers linked to the BB resistance genes in Y73 were scanned using the F population and the polymorphic markers. The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.

关键词： Oryza meyeriana     bacterial blight resistance gene     genetic population     linkage molecular marker    

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Transcriptome resources and genome-wide marker development for Japanese larch (

Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG

《农业科学与工程前沿（英文）》 2014年 第1卷 第1期   页码 77-84 doi: 10.15302/J-FASE-2014010

摘要： While the differential responses of trees to changes in climatic and environmental conditions have been demonstrated as they age, the underlying mechanisms and age control of tree growth and development are complex and poorly understood particularly at a molecular level. In this paper, we present a transcriptome analysis of , a deciduous conifer that is widely-grown in the northern hemisphere and of significant ecological and economic value. Using high-throughput RNA sequencing, we obtained about 26 million reads from the stems of 1-, 2-, 5-, 10-, 25- and 50-year-old trees. Combining these with the published Roche 454 sequencing reads and the expressed sequence tags (both mainly from embryogenic cell cultures), we assembled 26670549 reads into 146786 transcripts, of which we annotated 79182 to support investigations of the molecular basis of tree aging and adaption, somatic embryogenesis and wood formation. Using these sequences we also identified many single-nucleotide polymorphisms, simple sequence repeats, and insertion and deletion markers to assist breeding and genetic diversity studies of .

关键词： Larix     transcriptome     age     wood formation     somatic embryogenesis     molecular marker    

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Optimal design of a linkage–cam mechanism-based redundantly actuated parallel manipulator

《机械工程前沿（英文）》 2021年 第16卷 第3期   页码 451-467 doi: 10.1007/s11465-021-0634-6

摘要： A redundantly actuated parallel manipulator (RAPM) with mixed translational and rotational degrees of freedom (DOFs) is challenged for its dimensionally homogeneous Jacobian modeling and optimal design of architecture. In this paper, a means to achieve redundant actuation by adding kinematic constraints is introduced, which reduces the DOFs of the end-effector (EE). A generic dimensionally homogeneous Jacobian is developed for this type of RAPMs, which maps the generalized velocities of three points on the EE to the joint velocities. A new optimization algorithm derived from this dimensionally homogeneous Jacobian is proposed for the optimal design of this type of RAPMs. As an example, this paper presents a spatial RAPM involving linkages and cam mechanisms. This RAPM has 4 DOFs and 6 translational actuations. The linkage lengths and the position of the universal joints of the RAPM are optimized based on the dimensionally homogeneous Jacobian.

关键词： redundant actuation     parallel manipulator     linkage–cam mechanism     Jacobian     optimal design    

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The linkage mechanism between urban intelligence and low carbon innovation

Ming LIN

《工程管理前沿（英文）》 2019年 第6卷 第4期   页码 584-586 doi: 10.1007/s42524-019-0076-6

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Nonlinear dynamics and analysis of a four-bar linkage with clearance

Yuanguang TANG, Zongyu CHANG, Xiaogang DONG, Yafei HU, Zhenjiang YU

《机械工程前沿（英文）》 2013年 第8卷 第2期   页码 160-168 doi: 10.1007/s11465-013-0258-6

摘要：

In this paper, nonlinear dynamic behavior of a four-bar linkage considering clearance is studied. The dynamic model of the linkage with a clearance between coupler and rocker is developed firstly. Then the dynamic equations of this mechanism are solved by a numerical method. According to the calculated response, compliance, force and trajectory of pin in joint bearing are obtained. Effects of clearance magnitude and the relationship between a mechanism with clearance and without clearance are studied. By using Poincare Map, it is proved that strange attractors or chaos exist in the dynamic response. In addition, phenomena of chaos, periodic response and subharmonic response also can be found in the special condition. Bifurcation diagram is used to suggest that bifurcation and fractal phenomena exist in the dynamic response of this mechanism.

关键词： four-bar linkage     chaos     bifurcation     strange attractors     dynamics    

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“医养智慧联动”的养老模式研究与探索

金心宇,夏琦,张唯,李兰娟

《中国工程科学》 2018年 第20卷 第2期   页码 92-98 doi: 10.15302/J-SSCAE-2018.02.014

摘要：

我国已进入老龄化社会，而我国的养老建设事业仍处于不成熟阶段，养老问题已然成为我国亟待解决的重大问题之一。在国家相关政策的指引下，本文从我国国情出发，提出了一种以大型医院为诊疗主导，利用互联网实现与机构养老、社区养老和居家养老三种养老模式的有机连接，并融合老年病的人工智能云诊疗康复新技术的“医养智慧联动”养老模式。相关实践证明，利用互联网和人工智能及先进有效的综合诊疗技术来解决我国目前养老产业存在的问题具有重要的研究价值，也是一次非常有前景的尝试，符合国家的发展战略和相关政策。

关键词： “医养智慧联动”     智慧养老     互联网     人工智能     辅助诊疗    

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Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency

Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang

《医学前沿（英文）》 2020年 第14卷 第3期   页码 335-356 doi: 10.1007/s11684-019-0705-9

摘要： Sijunzi decoction (SJZD) is a Chinese classical formula to treat spleen qi deficiency syndrome (SQDS) and has been widely used for thousands of years. However, the quality control (QC) standards of SJZD are insufficient. Chinmedomics has been designed to discover and verify bioactive compounds of a variety of formula rapidly. In this study, we used Chinmedomics to evaluate the SJZD’s efficacy against SQDS to discover the potential quality-markers (q-markers) for QC. A total of 56 compounds in SJZD were characterized , and 23 compounds were discovered . A total of 58 biomarkers were related to SQDS, and SJZD can adjust a large proportion of marker metabolites to normal level and then regulate the metabolic profile to the health status. A total of 10 constituents were absorbed as effective ingredients that were associated with overall efficacy. We preliminarily determined malonyl-ginsenoside Rb2 and ginsenoside Ro as the q-markers of ginseng; dehydrotumulosic acid and dihydroxy lanostene-triene-21-acid as the q-markers of poria; glycyrrhizic acid, isoglabrolide, and glycyrrhetnic acid as the q-markers of licorice; and 2-atractylenolide as the q-marker of macrocephala. According to the discovery of the SJZD q-markers, we can establish the quality standard that is related to efficacy.

关键词： traditional Chinese medicine     Sijunzi decoction     spleen qi deficiency syndrome     Chinmedomics     quality-marker    

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Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma

null

《医学前沿（英文）》 2016年 第10卷 第1期   页码 52-60 doi: 10.1007/s11684-016-0433-3

摘要：

Hepatocellular carcinoma (HCC) is a lethal liver malignancy worldwide. In this study, we reported that protein phosphatase magnesium-dependent 1δ (PPM1D) was highly expressed in the majority of HCC cases (approximately 59%) and significantly associated with high serum α-fetoprotein (AFP) level (P= 0.044). Kaplan-Meier and Cox regression data indicated that PPM1D overexpression was an independent predictor of HCC-specific overall survival (HR, 2.799; 95% CI, 1.346–5.818, P = 0.006). Overexpressing PPM1D promoted cell viability and invasion, whereas RNA interference-mediated knockdown of PPM1D inhibited proliferation, invasion, and migration of cultured HCC cells. In addition, PPM1D suppression by small interfering RNA decreased the tumorigenicity of HCC cells in vivo. Overall, results suggest that PPM1D is a potential prognostic marker and therapeutic target for HCC.

关键词： PPM1D     hepatocellular carcinoma     prognosis     target therapy    

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Shaking-moment balancing of mechanisms with principal vectors and momentum

V. van der WIJK

《机械工程前沿（英文）》 2013年 第8卷 第1期   页码 10-16 doi: 10.1007/s11465-013-0359-2

摘要：

The design of shaking-moment-balanced linkages still is challenging. Considering moment balance in the very beginning of the design process of mechanisms is important for finding applicable solutions. For this purpose, the method of principal vectors is investigated, showing a compact notation of the angular momentum with respect to the center of mass. The moment balance conditions are derived for three elements in series from which balance solutions are synthesized and illustrated. From the application for moment balancing of a 4R four-bar linkage it is shown that the moment balancing of closed kinematic chains remains challenging.

关键词： shaking-moment balancing     principal vector linkage     angular momentum    

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Novel polymorphic EST-based microsatellite marker isolation and characterization from

Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG

《农业科学与工程前沿（英文）》 2015年 第2卷 第1期   页码 60-65 doi: 10.15302/J-FASE-2015048

摘要： Novel simple sequence repeat (SSR) markers were developed to evaluate their utility for genetic diversity and breeding studies of and related species. A total of 108 primer pairs were characterized by PCR amplification experiments. Among these, 61 were polymorphic and transferable to other citrus species. The number of alleles per locus ranged from 2 to 6, with an average of 2.37 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.83 and 0 to 1.00, respectively. These novel polymorphic SSR markers will be useful for citrus cultivar identification and evaluation as well as breeding studies.

关键词： microsatellite     polymorphism     Poncirus trifoliata    

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Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker Erratum

Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,

《工程（英文）》 2022年 第18卷 第11期   页码 259-259 doi: 10.1016/j.eng.2022.09.002

关键词： online     THE AUTHORS.Published     repertoire     behalf     Engineering     September     genotyping     Academy     HD-marker approach”     Education Press Limited Company.    

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Special issue on “Molecular Sensors and Molecular Logic Gates”

Luling Wu , Tony D. James

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 1-3 doi: 10.1007/s11705-021-2134-y

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Design of bio-oil additives via molecular signature descriptors using a multi-stage computer-aided molecular

《化学科学与工程前沿（英文）》 2022年 第16卷 第2期   页码 168-182 doi: 10.1007/s11705-021-2056-8

摘要： Direct application of bio-oil from fast pyrolysis as a fuel has remained a challenge due to its undesirable attributes such as low heating value, high viscosity, high corrosiveness and storage instability. Solvent addition is a simple method for circumventing these disadvantages to allow further processing and storage. In this work, computer-aided molecular design tools were developed to design optimal solvents to upgrade bio-oil whilst having low environmental impact. Firstly, target solvent requirements were translated into measurable physical properties. As different property prediction models consist different levels of structural information, molecular signature descriptor was used as a common platform to formulate the design problem. Because of the differences in the required structural information of different property prediction models, signatures of different heights were needed in formulating the design problem. Due to the combinatorial nature of higher-order signatures, the complexity of a computer-aided molecular design problem increases with the height of signatures. Thus, a multi-stage framework was developed by developing consistency rules that restrict the number of higher-order signatures. Finally, phase stability analysis was conducted to evaluate the stability of the solvent-oil blend. As a result, optimal solvents that improve the solvent-oil blend properties while displaying low environmental impact were identified.

关键词： computer-aided molecular design     bio-oil additives     molecular signature descriptor    

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Progress on molecular biomarkers and classification of malignant gliomas

null

《医学前沿（英文）》 2013年 第7卷 第2期   页码 150-156 doi: 10.1007/s11684-013-0267-1

摘要：

Gliomas are the most common primary intracranial tumors in adults. Anaplastic gliomas (WHO grade III) and glioblastomas (WHO grade IV) represent the major groups of malignant gliomas in the brain. Several diagnostic, predictive, and prognostic biomarkers for malignant gliomas have been reported over the last few decades, and these markers have made great contributions to the accuracy of diagnosis, therapeutic decision making, and prognosis of patients. However, heterogeneity in patient outcomes may still be observed, which highlights the insufficiency of a classification system based purely on histopathology. Great efforts have been made to incorporate new information about the molecular landscape of gliomas into novel classifications that may potentially guide treatment. In this review, we summarize three distinctive biomarkers, three most commonly altered pathways, and three classifications based on microarray data in malignant gliomas.

关键词： malignant glioma     molecular biomarker     IDH1     MGMT     molecular classification    

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Molecular classification and molecular targeted therapy of cancer

null

《医学前沿（英文）》 2013年 第7卷 第2期   页码 147-149 doi: 10.1007/s11684-013-0274-2

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标题 作者 时间 类型 操作

Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

期刊论文

Transcriptome resources and genome-wide marker development for Japanese larch (

Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG

期刊论文

Optimal design of a linkage–cam mechanism-based redundantly actuated parallel manipulator

期刊论文

The linkage mechanism between urban intelligence and low carbon innovation

Ming LIN

期刊论文

Nonlinear dynamics and analysis of a four-bar linkage with clearance

Yuanguang TANG, Zongyu CHANG, Xiaogang DONG, Yafei HU, Zhenjiang YU

期刊论文

“医养智慧联动”的养老模式研究与探索

金心宇,夏琦,张唯,李兰娟

期刊论文

Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency

Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang

期刊论文

Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma

null

期刊论文

Shaking-moment balancing of mechanisms with principal vectors and momentum

V. van der WIJK

期刊论文

Novel polymorphic EST-based microsatellite marker isolation and characterization from

Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG

期刊论文

Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker

Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,

期刊论文

Special issue on “Molecular Sensors and Molecular Logic Gates”

Luling Wu , Tony D. James

期刊论文

Design of bio-oil additives via molecular signature descriptors using a multi-stage computer-aided molecular

期刊论文

Progress on molecular biomarkers and classification of malignant gliomas

null

期刊论文

Molecular classification and molecular targeted therapy of cancer

null

期刊论文